Name | LEISHMAN'S STAIN |
Synonyms | LEISHMAN'S STAIN LeishmanSStainSolution LeishmanSStainForMicroscopy LEISHMANS EOSINE METHYLENE BLUE LEISHMANS EOSINE METHYLENE BLUE SOLUTION |
CAS | 12627-53-1 |
EINECS | 235-732-1 |
Molecular Formula | n.a. |
Water Solubility | Soluble in water (4 mg/ml), methanol (1 mg/ml), ethanol (4 mg/ml), and methyl cellosolve (20 mg/ml). |
Appearance | Morphological Powder |
Storage Condition | Store at +15°C to +30°C. |
MDL | MFCD00131498 |
Use | Use for stained blood smear examination to identify parasites, leukocytes and trypanosomes. |
Hazard Symbols | Xi - Irritant |
Risk Codes | R36/38 - Irritating to eyes and skin. R36/37/38 - Irritating to eyes, respiratory system and skin. |
Safety Description | S22 - Do not breathe dust. S24/25 - Avoid contact with skin and eyes. S36 - Wear suitable protective clothing. S26 - In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. |
WGK Germany | 3 |
HS Code | 32049000 |
application
ruite stain is used directly without any preparation process. trace neutral glycerol is added to the dye solution to prevent methanol from volatilization or oxidation, and at the same time, it can also make blood cell staining clearer. Often used for blood and cell smears, bone marrow cell smears, bacterial staining. The cytoplasm is red, the nucleus and bacteria are blue, and the eosinophilic particles are orange-red.
action
The staining of cells by Ritter stain has both physical adsorption and chemical affinity. The chemical properties of various cell components are different, and the affinity for various dyes is also different. Therefore, after dyeing with this dye solution, on the same blood film, you can see various colors, such as hemoglobin, eosinophilic particles are basic proteins, and the acidic dye eosin results, dyed pink, called eosinophilic Substances; nuclear protein and lymphocyte cytoplasm are acidic, combined with the basic dye methylene blue or azure, dyed purple blue, called basophilic substances; the neutral particles are in an isoelectric state and can be combined with eosin and blue. They are dyed lavender and are called neutral substances.